Determination of some microbial and chemical pollutants in cooling raw milk and ice-cream found in the local markets of Salahaldin province

Adil Abdulrahman Mustafa Ahmaed Ismaeal al-Nazal

Abstract

The study was conducted in the laboratories of food science Dept. College of agriculture- Tikrit University, which included the collection of samples of cooling raw milk and ice-cream of several trade markes and three replicates for each sample of different markets and areas in the province of salahaldin: Tikrit, Baiji, Sharqat and Alalam. The samples were taken to the laboratory, stored in the freezer at -18 0C until the test of microbial and chemical analysis. microbial analysis included the total bacterial count (T.B.C) of coliform & total bacterial count (T.B.C) of yeasts & molds also the isolation and diagnosis of listeria monocytogenes, and then conducted chemical analysis, wich included estimation of concentrations of four heavy metals: cadmium, lead, zinc and cupper. The results of microbiological tests showed that the total bacterial count (T.B.C) coliform, yeasts, molds and Liseria are:6.6,4.7,4.38,2.92 log10 cfu/g for ice-cream samples respectivaly, and 6.89,5.93,6.28,2.95 log10 cfu/g for cooling raw milk respectivaly. The results of the percentage of L.monocytogenes from the samples of ice-cream were 3.3% and cooling raw milk 18.75% .The results showed that L.monocytogenes was sensitive towards antibiotics: Ampicllin, Chloramphinicol , Erthromycin, Nitrofuranotion, Amikacin, and Tobbramycin,while it was resistant towards Naldix acid, Novoblocin and cefotaxame .The results showed also that the high concentration of heavy metals for cadimium, lead, zinc ,and copper found in ice-cream were 0.174, 0.17, 4.25, and 1.086 mg/g respectivily and for cooling raw milk samples were 0.1, 0.3, 2, 1.08 mg/lit respectivily .

Key words

Cooling raw milk,ice-cream, heavy metals, L.monocytogenes.